解剖学报

›› 2009, Vol. 40 ›› Issue (3): 401-404.doi: 10.3969/j.issn.0529-1356.2009.03.011

• 论著 • 上一篇    下一篇

枸橼酸铁铵对原代培养的大鼠心肌细胞铜蓝蛋白、膜铁转运辅助蛋白和膜铁转运蛋白表达的影响

王娜1，2 ; 关鹏1; 赵昭1 ;石欢欢1 ; 常彦忠1* ; 段相林1*    

  1. 1.河北师范大学生命科学学院，石家庄 050016;2.河北医科大学基础课部，石家庄 050091
  • 收稿日期:2008-02-07 修回日期:2008-04-23 出版日期:2009-06-06
  • 通讯作者: 常彦忠;段相林

Effects of ferric ammonium citrate on the expression of proteins related to iron release in primary cultured rat cardiac myocytes

  1. 1.College of Life Science, Hebei Normal University, Shijiazhuang 050016, China; 2.Department of Basic Courses, Hebei Medical University, Shijiazhuang 050091, China
  • Received:2008-02-07 Revised:2008-04-23 Online:2009-06-06
  • Contact: CHANG Yan-zhong;DUAN Xiang-lin

关键词: 枸橼酸铁铵, 心肌细胞, 铜蓝蛋白, 膜铁转运辅助蛋白, 膜铁转运蛋白, 免疫组织化学, 大鼠

Abstract: Objective To observe the effect of ferric ammonium citrate (FAC) on the pulsatile frequency and the expression of proteins related to iron releas in primary cultured rat cardiac myocytes. Methods Newborn rat cardiac myocytes were isolated and cultured with the medium containing FAC. The cells were divided into control group, 20mg/L FAC group, 40mg/L FAC group and 80mg/L FAC group. Every group had 6 repeats. The cells’ livability, beat rhythmically and beat amplitude of cardiac myocytes were detected. The expressions of ceruloplasmin (CP), hephaestin(HP) and ferroportion 1(FP1) were also examined by immunohistochemistry. Results Compared with control, FAC had no clearly influence on cells’ livability, but it could decrease cells’ beat rhythmically and beat amplitude. CP, HP and FP1 expression increased after the incubation of FAC. Conclusion FAC has no influence on cardiac myocytes’ livability, but it could affect the cells’ living state. IRP-IRE may be involved in the regulation of FP1 expression, the expression

Key words: Ferric ammonium citrate, Cardiac myocytes, Ceruloplasmin, Hephaestin, Ferroportin1, Immunohistochemistry, Rat

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